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multiscribe reverse transcriptase enzyme  (Thermo Fisher)


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    Structured Review

    Thermo Fisher multiscribe reverse transcriptase enzyme
    Multiscribe Reverse Transcriptase Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiscribe+reverse+transcriptase+enzyme/multiscribe+reverse+transcriptase+enzyme/pm39998652-43-19-23
    Average 90 stars, based on 1 article reviews
    multiscribe reverse transcriptase enzyme - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: MicroRNAs correlate with bacillary index and genes associated to cell death processes in leprosy.
    Article Snippet: Mycobacterium leprae infects skin and peripheral nerves causing a broad of clinical forms.. MicroRNAs (miRNAs) control immune mechanisms such as apoptosis, autophagy as well as to target genes leading to abnormal proliferation, metastasis, and invasion of cells.. Herein we evaluated miRNAs expression for leprosy phenotypes in biopsies obtained from patients with and without reactions.

    Article Title: Role of LCN2 in a murine model of hindlimb ischemia and in peripheral artery disease patients, and its potential regulation by miR-138-5P.
    Article Snippet: 1 μg of total RNA was reverse transcribed with random primers and Moloney murine leukemia virus reverse transcriptase (Thermo Fisher Scientific). .. For miRNAs, 5 ng of total RNA was retro-transcribed with stem-loop reverse transcription primers (Thermo Fisher Scientific) and MultiScribe reverse transcriptase enzyme (Thermo Fisher Scientific). qPCRs were performed on a ViiA 7 Real-Time PCR System (Thermo Fisher Scientific) using PrimeTime qPCR gene expression assays (IDT) for the protein-coding transcripts and TaqMan® MicroRNA Assays (Thermo Fisher Scientific) for the miRNAs (Supplemental Table 1). β-actin and GAPDH were used as housekeeping genes for mRNA analysis in mouse and human samples respectively. ..

    Article Title: Soluble isoforms of the DC-SIGN receptor can increase the dengue virus infection in immature dendritic cells.
    Article Snippet: The viral RNA was quantified in an absorbance spectrophotometer and submitted to the RT-PCR in a thermocycler (AB-Applied Biosystem, Veriti thermal cycler, 2010). .. The cDNA production was performed from approximately 1 ug of RNA, 10£ Random Primer, 10 mM dNTP’s, Depc water, 10£ RT enzyme buffer and the MultiScribe Reverse Transcriptase enzyme with a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, USA). ..

    Article Title: Clinical and Neurodevelopmental Characteristics of Enterovirus and Parechovirus Meningitis in Neonates
    Article Snippet: .. The targeted region of the viral RNA sequence was reverse transcribed to single stranded complementary DNA (cDNA) via primer extension with the Multiscribe Reverse Transcriptase enzyme (Thermofisher Scientific) on Gene Amp PCR System 9,700 (Applied Biosystem), using four different primers. .. Amplifications were carried on with AmpliTaq GoldTM DNA Polymerases (Applied Biosystem) on Gene Amp PCR System 9,700, using two sets of primers.

    Article Title: Investigation of MicroRNA-134 as a Target against Seizures and SUDEP in a Mouse Model of Dravet Syndrome
    Article Snippet: The quantity and quality of RNA were measured using a Nanodrop Spectrophotometer (Thermo Fisher Scientific). .. Samples with a 260/280 ratio between 1.8 and 2.2 were considered acceptable; 250ng of total RNA was used to produce cDNA by reverse transcription using Multiscribe reverse transcriptase enzyme (Invitrogen). ..

    Article Title: Association of TOLLIP mRNA Expression and TOLLIP rs5743899A/G, TOLLIP rs3750920C/T, and IL-10 -1082G/A Single-Nucleotide Polymorphisms with Susceptibility to Tuberculosis.
    Article Snippet: The early innate host immune response is crucial in the susceptibility to tuberculosis (TB).. This study aimed to investigate the potential role of Toll-interacting protein (TOLLIP), a negative regulator of the human innate immune response, and interleukin-10 (IL-10), an anti-inflammatory cytokine, in susceptibility to TB.. The peripheral blood mononuclear cells of 30 active pulmonary TB patients and 30 healthy volunteers were included.

    Article Title: MicroRNAs regulating macrophages infected with Leishmania L. ( V. ) Braziliensis isolated from different clinical forms of American tegumentary leishmaniasis
    Article Snippet: TaqManTM assays containing specific primers and probes for the BCL2 (Hs01048932_g1), CFLAR (Hs01048932_g1), ATG12 , (Hs00269492_m1), GZMB (Hs00188051_m1), and TNFRSF10A (Hs00269492_m1) genes were purchased from Thermo Fisher Scientific as well as the normalizer for the ACTB gene (Hs99999903_m1). .. Reverse transcription reactions were performed using the commercially available High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific), starting from 10μl of total RNA and using the MultiScribe Reverse Transcriptase enzyme, according to the manufacturer’s instructions. .. Gene expression analysis was performed using the qPCR technique with the QuantStudio 3 (Applied Biosystems ® ).

    Real-time Polymerase Chain Reaction:

    Article Title: Role of LCN2 in a murine model of hindlimb ischemia and in peripheral artery disease patients, and its potential regulation by miR-138-5P.
    Article Snippet: 1 μg of total RNA was reverse transcribed with random primers and Moloney murine leukemia virus reverse transcriptase (Thermo Fisher Scientific). .. For miRNAs, 5 ng of total RNA was retro-transcribed with stem-loop reverse transcription primers (Thermo Fisher Scientific) and MultiScribe reverse transcriptase enzyme (Thermo Fisher Scientific). qPCRs were performed on a ViiA 7 Real-Time PCR System (Thermo Fisher Scientific) using PrimeTime qPCR gene expression assays (IDT) for the protein-coding transcripts and TaqMan® MicroRNA Assays (Thermo Fisher Scientific) for the miRNAs (Supplemental Table 1). β-actin and GAPDH were used as housekeeping genes for mRNA analysis in mouse and human samples respectively. ..

    Gene Expression:

    Article Title: Role of LCN2 in a murine model of hindlimb ischemia and in peripheral artery disease patients, and its potential regulation by miR-138-5P.
    Article Snippet: 1 μg of total RNA was reverse transcribed with random primers and Moloney murine leukemia virus reverse transcriptase (Thermo Fisher Scientific). .. For miRNAs, 5 ng of total RNA was retro-transcribed with stem-loop reverse transcription primers (Thermo Fisher Scientific) and MultiScribe reverse transcriptase enzyme (Thermo Fisher Scientific). qPCRs were performed on a ViiA 7 Real-Time PCR System (Thermo Fisher Scientific) using PrimeTime qPCR gene expression assays (IDT) for the protein-coding transcripts and TaqMan® MicroRNA Assays (Thermo Fisher Scientific) for the miRNAs (Supplemental Table 1). β-actin and GAPDH were used as housekeeping genes for mRNA analysis in mouse and human samples respectively. ..

    Article Title: Association of TOLLIP mRNA Expression and TOLLIP rs5743899A/G, TOLLIP rs3750920C/T, and IL-10 -1082G/A Single-Nucleotide Polymorphisms with Susceptibility to Tuberculosis.
    Article Snippet: The early innate host immune response is crucial in the susceptibility to tuberculosis (TB).. This study aimed to investigate the potential role of Toll-interacting protein (TOLLIP), a negative regulator of the human innate immune response, and interleukin-10 (IL-10), an anti-inflammatory cytokine, in susceptibility to TB.. The peripheral blood mononuclear cells of 30 active pulmonary TB patients and 30 healthy volunteers were included.

    cDNA Synthesis:

    Article Title: Enhancing cell death in B-cell malignancies through targeted inhibition of Bcl-3
    Article Snippet: RNA concentrations were measured using a NanoDrop 2000 spectrophotometer (Thermo Scientific). .. The cDNA synthesis was performed using 10X RT random primers, dNTP mix, 10X RT buffer and Multiscribe Reverse Transcriptase enzyme (Applied Biosystems). .. The amplifications were run on a T Gradient Thermoblock PCR Thermocycler (Biometra).

    Sequencing:

    Article Title: Clinical and Neurodevelopmental Characteristics of Enterovirus and Parechovirus Meningitis in Neonates
    Article Snippet: .. The targeted region of the viral RNA sequence was reverse transcribed to single stranded complementary DNA (cDNA) via primer extension with the Multiscribe Reverse Transcriptase enzyme (Thermofisher Scientific) on Gene Amp PCR System 9,700 (Applied Biosystem), using four different primers. .. Amplifications were carried on with AmpliTaq GoldTM DNA Polymerases (Applied Biosystem) on Gene Amp PCR System 9,700, using two sets of primers.

    Polymerase Chain Reaction:

    Article Title: Clinical and Neurodevelopmental Characteristics of Enterovirus and Parechovirus Meningitis in Neonates
    Article Snippet: .. The targeted region of the viral RNA sequence was reverse transcribed to single stranded complementary DNA (cDNA) via primer extension with the Multiscribe Reverse Transcriptase enzyme (Thermofisher Scientific) on Gene Amp PCR System 9,700 (Applied Biosystem), using four different primers. .. Amplifications were carried on with AmpliTaq GoldTM DNA Polymerases (Applied Biosystem) on Gene Amp PCR System 9,700, using two sets of primers.



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